ATP-dependent accumulation and inositol trisphosphate- or cyclic ADP-ribose-mediated release of Ca2+ from the nuclear envelope

Cell. 1995 Feb 10;80(3):439-44. doi: 10.1016/0092-8674(95)90494-8.


Uptake and release of Ca2+ from isolated liver nuclei were studied with fluorescent probes. We show with the help of digital imaging and confocal microscopy that the Ca(2+)-sensitive fluorescent probe Fura 2 is concentrated in or around the nuclear envelope and that the distribution of Fura 2 fluorescence is similar to that of an endoplasmic reticulum marker. The previously demonstrated ATP-dependent uptake of Ca2+ into isolated nuclei and release of the accumulated Ca2+ by inositol 1,4,5-trisphosphate (IP3) are therefore due to transport of Ca2+ into and out of the nuclear envelope and not the nucleoplasm. Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration. Nevertheless, IP3 and cyclic ADP-ribose evoke transient intranuclear Ca2+ elevations. The release from the Ca2+ stores in or around the nuclear envelope appears to be directed into the nucleoplasm from where it can diffuse out through the permeable nuclear pore complexes.

MeSH terms

  • Adenosine Diphosphate Ribose / analogs & derivatives*
  • Adenosine Diphosphate Ribose / pharmacology
  • Adenosine Triphosphate / physiology*
  • Calcium / metabolism*
  • Carbocyanines
  • Cyclic ADP-Ribose
  • Endoplasmic Reticulum / metabolism
  • Fluorescent Dyes
  • Fura-2
  • Inositol 1,4,5-Trisphosphate / pharmacology*
  • Ion Transport / drug effects
  • Liver / cytology
  • Liver / metabolism
  • Microscopy, Confocal
  • Models, Biological
  • Nuclear Envelope / metabolism*
  • Ryanodine / pharmacology


  • Carbocyanines
  • Fluorescent Dyes
  • Cyclic ADP-Ribose
  • Ryanodine
  • Adenosine Diphosphate Ribose
  • 3,3'-dihexyl-2,2'-oxacarbocyanine
  • Inositol 1,4,5-Trisphosphate
  • Adenosine Triphosphate
  • Calcium
  • Fura-2