The SH2-containing protein-tyrosine phosphatase SH-PTP2 is required upstream of MAP kinase for early Xenopus development

Cell. 1995 Feb 10;80(3):473-83. doi: 10.1016/0092-8674(95)90498-0.

Abstract

SH-PTP2, the vertebrate homolog of Drosophila corkscrew, associates with several activated growth factor receptors, but its biological function is unknown. We assayed the effects of injection of wild-type and mutant SH-PTP2 RNAs on Xenopus embryogenesis. An internal phosphatase domain deletion (delta P) acts as a dominant negative mutant, causing severe posterior truncations. This phenotype is rescued by SH-PTP2, but not by the closely related SH-PTP1. In ectodermal explants, delta P blocks fibroblast growth factor (FGF)- and activin-mediated induction of mesoderm and FGF-induced mitogen-activated protein (MAP) kinase activation. Our results indicate that SH-PTP2 is required for early vertebrate development, acting as a positive component in FGF signaling downstream of the FGF receptor and upstream of MAP kinase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activins
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cloning, Molecular
  • Culture Techniques
  • Ectoderm / drug effects
  • Ectoderm / physiology*
  • Embryonic Induction* / drug effects
  • Female
  • Fibroblast Growth Factor 2 / pharmacology
  • Inhibins / pharmacology
  • Intracellular Signaling Peptides and Proteins
  • Mesoderm / drug effects
  • Mesoderm / physiology*
  • Molecular Sequence Data
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases / genetics
  • Protein Tyrosine Phosphatases / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / pharmacology
  • Sequence Analysis, DNA
  • Sequence Deletion / physiology
  • Xenopus

Substances

  • Intracellular Signaling Peptides and Proteins
  • RNA, Messenger
  • Fibroblast Growth Factor 2
  • Activins
  • Inhibins
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases

Associated data

  • GENBANK/U15287