Nucleotide sequence analysis of a full-length human T-cell leukemia virus type I from adult T-cell leukemia cells: a prematurely terminated PX open reading frame II

Int J Cancer. 1995 Mar 3;60(5):701-6. doi: 10.1002/ijc.2910600522.


Human T-cell leukemia virus type 1 (HTLV-1) is etiologically associated with adult T-cell leukemia/lymphoma (ATL). The prototypic HTLV-1, ATK, is the only full-length provirus cloned from uncultured leukemic cells and completely sequenced prior to this study. We have determined the complete nucleotide sequence of another full-length HTLV-1 provirus cloned directly from leukemic cells. A premature termination codon was found in the second open reading frame (orf II) of the pX region. Our finding indicates that open reading frame II of the HTLV-1 pX region is not required for outgrowth of ATL leukemic clones in vivo.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Neoplasm / genetics
  • DNA, Viral / genetics
  • Human T-lymphotropic virus 1 / genetics*
  • Human T-lymphotropic virus 1 / isolation & purification
  • Humans
  • Leukemia-Lymphoma, Adult T-Cell / genetics
  • Leukemia-Lymphoma, Adult T-Cell / virology*
  • Molecular Sequence Data
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Proviruses / genetics
  • RNA Splicing
  • Sequence Alignment
  • Sequence Deletion*
  • Sequence Homology
  • Terminator Regions, Genetic*


  • DNA, Neoplasm
  • DNA, Viral

Associated data

  • GENBANK/U19949