Interferon expression in Crohn's disease patients: increased interferon-gamma and -alpha mRNA in the intestinal lamina propria mononuclear cells

J Interferon Res. 1994 Oct;14(5):235-8. doi: 10.1089/jir.1994.14.235.


The in vivo interferon (IFN) activation in Crohn's disease was evaluated by measuring the relative amounts of IFN-alpha and -gamma mRNA in freshly isolated human lamina propria mononuclear cells (LPMC) from patients with Crohn's disease and controls. Both IFN-gamma and IFN-alpha mRNA, as estimated by dot blot analysis, were increased in Crohn's disease (LPMC), although the relative amounts of IFN mRNA appeared to differ among patients. Appreciable amounts of IFN-gamma mRNA were found in Crohn's disease peripheral blood mononuclear cells (PBMC) extracts, whereas the same cells were negative for IFN-alpha mRNA. Only minute amounts of IFN-gamma RNA were found sporadically in control LPMC while no IFN-alpha was detected. Control PBMC were shown to be virtually negative for both IFN-alpha and IFN-gamma mRNA. These data suggest that IFN induction in the normal human gut is a well-controlled function and that in Crohn's disease tissues, both IFN-gamma and IFN-alpha production are dysregulated. The increased IFN activity may represent a major feature in the induction and perpetuation of the chronic inflammatory process in Crohn's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crohn Disease / metabolism*
  • Evaluation Studies as Topic
  • Humans
  • Interferon-alpha / genetics*
  • Interferon-gamma / genetics*
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / metabolism*
  • Leukocytes, Mononuclear / metabolism*
  • RNA, Messenger / biosynthesis*


  • Interferon-alpha
  • RNA, Messenger
  • Interferon-gamma