Reactivity of serum anti-erythrocyte membrane antibody in Babesia gibsoni-infected dogs

J Vet Med Sci. 1994 Oct;56(5):997-9. doi: 10.1292/jvms.56.997.


Reactivity of anti-erythrocyte membrane (RBCm) antibody in sera of B. gibosni-infected dogs on native RBCm and erythrocyte surface (RBCs) was examined using an enzyme linked immunosorbent assay. Anti-RBCm antibody attached to native RBCm and the surface of erythrocytes (RBC) treated with phenylhydrazine or neuraminidase, but not to the surface of non-treated RBC. Based on these results, anti-cytoskeletal protein antibody in sera of B. gibsoni-infected dogs is considered to attach to native RBCm, and furthermore free radical-induced oxidative stress imposed on RBC and sialic acid removal from glycoproteins of RBC are considered necessary for anti-transmembrane protein antibody in sera of B. gibsoni-infected dogs to be bound to RBCs. These are important to elucidating the mechanism of the marked increase in RBCs-bound IgG value and anemia in B. gibsoni infection.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Protozoan / blood*
  • Antigen-Antibody Reactions
  • Autoantibodies / blood*
  • Babesiosis / blood
  • Babesiosis / immunology*
  • Cytoskeletal Proteins / immunology
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Erythrocyte Membrane / drug effects
  • Erythrocyte Membrane / immunology*
  • Free Radicals
  • Neuraminidase / pharmacology
  • Phenylhydrazines / pharmacology


  • Antibodies, Protozoan
  • Autoantibodies
  • Cytoskeletal Proteins
  • Free Radicals
  • Phenylhydrazines
  • phenylhydrazine
  • Neuraminidase