Lack of direct action of D-fenfluramine on arcuate preproNPY mRNA levels in the rat

Neuroreport. 1994 Oct 27;5(16):2113-6. doi: 10.1097/00001756-199410270-00031.

Abstract

Hypothalamic neuropeptide Y (NPY) and 5-hydroxytryptamine (5-HT) may interact to regulate food intake. This study investigated the effects of the 5-HT reuptake blocker and secretagogue D-fenfluramine (D-FEN) on preproNPY mRNA abundance in the hypothalamic arcuate nucleus (ARC) of free-feeding and food-deprived (FD) rats in two treatment regimens. ARC preproNPY mRNA levels were significantly increased in FD rats, to the same extent as in combined FD/D-FEN animals. D-FEN treatments producing different-sized reductions in food intake (and body weight) produced corresponding changes in ARC preproNPY mRNA abundance. These findings suggest that ARC preproNPY mRNA levels are not directly altered by D-FEN, and that D-FEN-induced anorexia does not involve effects on the hypothalamic NPY system at the level of NPY gene transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arcuate Nucleus of Hypothalamus / drug effects*
  • Arcuate Nucleus of Hypothalamus / metabolism
  • Body Weight / drug effects
  • Eating / drug effects
  • Fenfluramine / pharmacology*
  • Male
  • Neuropeptide Y / genetics*
  • Protein Precursors / genetics*
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Neuropeptide Y
  • Protein Precursors
  • RNA, Messenger
  • Fenfluramine
  • preproneuropeptide Y