Regulation of CD40 ligand expression and use of recombinant CD40 ligand for studying B cell growth and differentiation

Semin Immunol. 1994 Oct;6(5):287-94. doi: 10.1006/smim.1994.1037.

Abstract

Helper T cell activation leads to transient expression of a ligand for the B cell surface protein, CD40. CD40 ligand can deliver helper T cell-derived contact signals to B lymphocytes that drive B cell activation and proliferation. Regulation of expression of CD40 ligand is analogous to that of other helper T cell-derived lymphokines. A soluble form of CD40 ligand is capable of delivering proliferative signals to B cells only when cross-linked or when IL-4 is added. Recombinant CD40 ligand in membrane vesicles can be used to develop in vitro systems for studying class switching, clonal selection, and affinity maturation in normal B lymphocytes.

Publication types

  • Review

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • CD40 Ligand
  • Cell Differentiation / immunology
  • Gene Expression Regulation / immunology*
  • Humans
  • Lymphocyte Activation / immunology*
  • Membrane Glycoproteins / biosynthesis*
  • Membrane Glycoproteins / immunology*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / immunology
  • T-Lymphocytes, Helper-Inducer / immunology

Substances

  • Membrane Glycoproteins
  • Recombinant Proteins
  • CD40 Ligand