Molecular characterization of a cytoplasmic dynein from Dictyostelium

J Eukaryot Microbiol. Nov-Dec 1994;41(6):645-51. doi: 10.1111/j.1550-7408.1994.tb01528.x.

Abstract

Cytoplasmic dynein is a high molecular weight, microtubule-based mechanochemical ATPase that is believed to provide motive force for a number of intracellular motilities, including transport of membrane-bound organelles. Cytoplasmic dynein also localizes to the mitotic spindles of some organisms and to the kinetochore regions of some condensed chromosomes, where it may play an active role in spindle assembly, spindle position, and/or chromosome movement during cell division. Despite active research efforts from a number of laboratories, little detail is yet available about dynein-based cellular activities. This paper describes our efforts to characterize cytoplasmic dynein from Dictyostelium and to use this protist as a molecular genetic factory to probe structure-function relationships of this molecule.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cytoplasm / enzymology
  • Dictyostelium / enzymology*
  • Dictyostelium / genetics
  • Dyneins / chemistry
  • Dyneins / genetics*
  • Dyneins / isolation & purification
  • Dyneins / ultrastructure
  • Gene Expression Regulation, Enzymologic / genetics*
  • Genes, Protozoan / genetics
  • Genetic Vectors / genetics
  • Molecular Sequence Data
  • Molecular Weight
  • Promoter Regions, Genetic / genetics
  • Sequence Homology, Amino Acid
  • Transformation, Genetic

Substances

  • Dyneins

Associated data

  • GENBANK/X79678