Cross Reactivity Studies of CRF-related Peptides on Insect Malpighian Tubules

Comp Biochem Physiol A Physiol. 1995 Jan;110(1):87-93. doi: 10.1016/0300-9629(94)00132-d.


Manduca sexta diuretic peptide II (Mas-DPII) stimulates fluid secretion by adult Malpighian tubules and cyclic AMP production by larval proximal and adult tubules of M. sexta in a dose-dependent manner. Mas-DPII has no effect on fluid transport across the larval cryptonephric complex. M. sexta diuretic hormone (Mas-DH) and CRF-related insect diuretic peptides from Acheta domesticus, Locusta migratoria, and Periplaneta americana also cause similar increases in the production of cyclic AMP by the Malpighian tubules of both larval and adult M. sexta. Insect CRF-related diuretic peptides exhibit varying degrees of potency when assayed on Malpighian tubules from L. migratoria and A. domesticus. Sauvagine, bovine-CRF, and human-CRF have only a small, but significant, effect on cyclic AMP production by M. sexta Malpighian tubules. However, sauvagine, bovine-CRF, and sucker fish urotensin-I have no effect on L. migratoria tubules. Stimulation of cyclic AMP production by M. sexta Malpighian tubules could potentially be used as a screening assay to identify other insect CRF-related diuretic peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Amino Acid Sequence
  • Animals
  • Biological Transport / drug effects
  • Body Fluids / metabolism
  • Corticotropin-Releasing Hormone / genetics
  • Corticotropin-Releasing Hormone / pharmacology*
  • Cyclic AMP / biosynthesis
  • Diuretics / pharmacology
  • Female
  • Insect Hormones / pharmacology
  • Insecta / growth & development
  • Insecta / physiology*
  • Intercellular Signaling Peptides and Proteins
  • Larva
  • Malpighian Tubules / drug effects*
  • Malpighian Tubules / metabolism
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / pharmacology*
  • Peptides*
  • Vertebrates / metabolism


  • Diuretics
  • Insect Hormones
  • Intercellular Signaling Peptides and Proteins
  • Peptide Fragments
  • Peptides
  • Mas-DP II
  • Corticotropin-Releasing Hormone
  • Cyclic AMP