84 suspected patients with gonococcal urethritis were diagnosed by polymerase chain reaction (PCR). It was demonstrated that 28 patients (33.3%) were positive, and 56 (66.7%) negative. Of the PCR-positive patients, 18 showed secretions from the urethral tract, on which the bacterial stain and culture were carried out. The results indicate that 12 patients were positive, and 6 negative. There was no patient with positive stain and culture with PCR-negative. We conclude that PCR is more specific and sensitive than other routine methods in the diagnosis of gonococcal urethritis, especially of chronic infection.