Function of Protein Kinase A in Hedgehog Signal Transduction and Drosophila Imaginal Disc Development

Cell. 1995 Feb 24;80(4):553-62. doi: 10.1016/0092-8674(95)90509-x.

Abstract

Reduced protein kinase A (PKA) activity in anterior imaginal disc cells leads to cell-autonomous induction of decapentaplegic (dpp), wingless (wg), and patched (ptc) transcription that is independent of hedgehog (hh) gene activity. The resulting nonautonomous adult wing and leg pattern duplications are largely due to induced dpp and wg expression and resemble phenotypes elicited by ectopic hh expression. Inhibition of PKA in anterior cells close to the posterior compartment can substitute for hh activity to promote growth of imaginal discs, whereas overexpression of PKA can counteract transcriptional induction of ptc by hh in these cells. PKA therefore appears to be an integral component of the mechanism by which hh regulates the expression of key patterning molecules in imaginal discs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Congenital Abnormalities / genetics
  • Cyclic AMP-Dependent Protein Kinases / genetics
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Drosophila / embryology*
  • Drosophila Proteins*
  • Female
  • Gene Expression Regulation*
  • Hedgehog Proteins
  • Insect Hormones / biosynthesis
  • Insect Hormones / genetics
  • Larva
  • Male
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics
  • Mice
  • Models, Genetic
  • Mutation
  • Protein Biosynthesis*
  • Proteins / antagonists & inhibitors
  • Proteins / genetics
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins / genetics
  • Receptors, Cell Surface
  • Recombinant Proteins / biosynthesis
  • Signal Transduction*
  • Transcription, Genetic
  • Wings, Animal / embryology
  • Wnt1 Protein

Substances

  • Drosophila Proteins
  • Hedgehog Proteins
  • Insect Hormones
  • Membrane Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • Receptors, Cell Surface
  • Recombinant Proteins
  • Wnt1 Protein
  • dpp protein, Drosophila
  • ptc protein, Drosophila
  • wg protein, Drosophila
  • hh protein, Drosophila
  • Cyclic AMP-Dependent Protein Kinases