Interleukin-10 Downregulates Protective Immunity to Brucella Abortus

Infect Immun. 1995 Mar;63(3):1130-3.

Abstract

In vivo neutralization of interleukin-10 (IL-10) with an anti-IL-10 monoclonal antibody resulted in up to 10-fold fewer bacteria in the spleens of BALB/c mice infected with the virulent Brucella abortus strain 2308. In vitro neutralization of endogenous IL-10 in brucella antigen-stimulated cultures of splenocytes from infected mice resulted in increased gamma interferon production in these cultures, whereas exogenous recombinant IL-10 inhibited the ability of peptone-elicited peritoneal macrophages to control intracellular brucellae. These data suggest that IL-10 may be downregulating the immune response to B. abortus by affecting both macrophage effector function and the production of the protective Th1 cytokine gamma interferon.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • Brucella abortus / immunology*
  • Brucellosis / immunology
  • Brucellosis / prevention & control*
  • Chronic Disease
  • Down-Regulation
  • Female
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / immunology*
  • Macrophages / immunology
  • Mice
  • Mice, Inbred BALB C
  • Neutralization Tests
  • Spleen / cytology
  • Spleen / immunology
  • Th2 Cells / immunology

Substances

  • Interleukin-10
  • Interferon-gamma