AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells

J Immunol. 1995 Mar 1;154(5):2012-22.

Abstract

Interactions between T cells and epithelial cells of the thymus are essential for normal T cell development, and interactions between T cells and skin epidermal keratinocytes occur in the context of inflammatory skin diseases and cutaneous T cell malignancies. On the basis of observations that the T cell ALL cell line, HSB, bound to IFN-gamma activated epidermal keratinocytes (41 +/- 5% of EK with three or more HSB cells bound), whereas the CTCL cell line H9 bound poorly (8 +/- 3%), we have raised mAb 13H12 that identified a 36 kDa molecule, termed AD2, that was highly expressed on HSB but not on H9 T cells. mAb 13H12 inhibited the binding of HSB T cells to IFN-gamma-treated epidermal keratinocytes (43 +/- 5% inhibition, p < 0.01), inhibited the binding of peripheral blood T cells to IFN-gamma-treated EK (62 +/- 3% inhibition, p < 0.001), and inhibited the binding of IFN-gamma-treated human thymic epithelial cells to thymocytes (39 +/- 3% inhibition, p < 0.01). Although AD2 was expressed at a low level on all T cells, AD2 was highly expressed on the CD3-CD4-CD8-, CD3-CD4low+ CD8-, and CD3-CD4+ CD8+ subsets of immature thymocytes in the thymic subcapsular and inner cortex. Taken together, these data suggest a role for the AD2 molecule in interactions of T cells with epithelial cells of skin and thymus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Antigens, Differentiation, T-Lymphocyte / metabolism
  • Cell Adhesion / immunology
  • Cell Adhesion / physiology
  • Cell Line
  • Epithelial Cells
  • Humans
  • Interferon-gamma / metabolism
  • Keratinocytes / immunology
  • Keratinocytes / physiology*
  • Membrane Proteins / immunology
  • Membrane Proteins / physiology
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / physiology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*
  • Thymus Gland / cytology*
  • Thymus Gland / immunology
  • Thymus Gland / physiology

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Membrane Proteins
  • Interferon-gamma