Isolation of nine gene sequences induced by silica in murine macrophages

J Immunol. 1995 Mar 1;154(5):2384-92.


Macrophage activation by silica is the initial step in the development of silicosis. To identify genes that might be involved in silica-mediated activation, RAW 264.7 mouse macrophages were treated with silica for 48 h, and a subtracted cDNA library enriched for silica-induced genes (SIG) was constructed and differentially screened. Nine cDNA clones (designated SIG-12, -14, -20, -41, -61, -81, -91, -92, and -111) were partially sequenced and compared with sequences in GenBank/EMBL databases. SIG-12, -14, and -20 corresponded to the genes for ribosomal proteins L13a, L32, and L26, respectively. SIG-61 is the mouse homologue of p21 RhoC. SIG-91 is identical to the 67-kDa high-affinity laminin receptor. Four genes were not identified and are novel. All of the mRNAs corresponding to the nine cloned cDNAs were inducible by silica. Steady-state levels of mRNAs in RAW 264.7 cells treated with various macrophage activators and inducers of signal transduction pathways were determined. A complex pattern of induction and repression was found, indicating that upon phagocytosis of silica particles, many regulatory mechanisms of gene expression are simultaneously triggered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression Regulation / drug effects*
  • Gene Library
  • Macrophage Activation / drug effects*
  • Macrophage Activation / genetics*
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Silicon Dioxide / toxicity*
  • Silicosis / etiology
  • Silicosis / genetics


  • DNA, Complementary
  • RNA, Messenger
  • Silicon Dioxide

Associated data

  • GENBANK/X80232
  • GENBANK/X80638
  • GENBANK/X80699
  • GENBANK/X80899
  • GENBANK/X81443
  • GENBANK/X81444