Detection of west Nile and Japanese encephalitis viral genome sequences in cerebrospinal fluid from acute encephalitis cases in Karachi, Pakistan

Microbiol Immunol. 1994;38(10):827-30. doi: 10.1111/j.1348-0421.1994.tb01866.x.


Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Base Sequence
  • Cerebrospinal Fluid / virology
  • Child
  • Child, Preschool
  • DNA Primers / chemistry
  • DNA, Viral / analysis
  • Encephalitis Virus, Japanese / genetics*
  • Encephalitis Virus, Japanese / isolation & purification
  • Encephalitis, Japanese / cerebrospinal fluid*
  • Encephalitis, Japanese / virology*
  • Female
  • Genome, Viral*
  • Humans
  • Infant
  • Male
  • Molecular Sequence Data
  • West Nile Fever / cerebrospinal fluid*
  • West Nile Fever / virology*
  • West Nile virus / genetics*
  • West Nile virus / isolation & purification


  • DNA Primers
  • DNA, Viral