Positive correlation between H,K-adenosine triphosphatase autoantibodies and Helicobacter pylori antibodies in patients with pernicious anemia

Scand J Gastroenterol. 1994 Nov;29(11):961-5. doi: 10.3109/00365529409094870.


Background: Helicobacter pylori is a major cause of gastritis, and the parietal cell H,K-adenosine triphosphatase (ATPase) is a major autoantigen in autoimmune atrophic corpus gastritis, which may eventually lead to pernicious anemia and/or neuropathy. Whether the bacterium induces the autoimmune response is unknown.

Methods: By means of enzyme-linked immunosorbent assay the occurrence of antibodies against porcine H,K-ATPase and H. pylori was determined in sera from 30 patients with pernicious anemia.

Results: All sera scored positive against H,K-ATPase, and 25 (83%) scored positive against H. pylori. The titers of antibodies against both antigen preparations inversely correlated with the duration of disease. A possible common epitope in the antigen preparations was tested with a competition assay. There was no indication of a common epitope in either human or porcine H,K-ATPase and H. pylori.

Conclusions: There was a positive correlation and a high incidence of antibodies against H,K-ATPase and H. pylori in sera from patients with pernicious anemia. These antibodies recognized different epitopes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Anemia, Pernicious / immunology*
  • Anemia, Pernicious / microbiology
  • Animals
  • Antibodies, Bacterial / immunology*
  • Autoantibodies / immunology*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Female
  • Gastritis, Atrophic / etiology
  • Gastritis, Atrophic / immunology*
  • H(+)-K(+)-Exchanging ATPase / immunology*
  • Helicobacter Infections / immunology*
  • Helicobacter pylori / immunology*
  • Humans
  • Male
  • Swine


  • Antibodies, Bacterial
  • Autoantibodies
  • H(+)-K(+)-Exchanging ATPase