Nucleotide sequence of a cDNA for mouse squalene epoxidase

Biochim Biophys Acta. 1995 Feb 21;1260(3):345-8. doi: 10.1016/0167-4781(94)00245-x.

Abstract

A full-length cDNA encoding mouse squalene epoxidase was isolated by screening a mouse liver cDNA library with the rat squalene epoxidase gene as a probe. The cDNA had an open reading frame for a 572 amino acid polypeptide with a calculated molecular mass of 63.8 kDa. The predicted amino acid sequence of the mouse enzyme contained an FAD-binding motif, and was 93% identical to that of the rat enzyme. The former is one amino acid shorter than the latter. Blotting analyses showed that the mRNA is 2.8 kb in size and that a single copy of the gene is present in the mouse genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary
  • Mice
  • Molecular Sequence Data
  • Oxygenases / chemistry
  • Oxygenases / genetics*
  • Sequence Homology, Amino Acid
  • Squalene Monooxygenase

Substances

  • DNA, Complementary
  • Oxygenases
  • Squalene Monooxygenase

Associated data

  • GENBANK/D42048