Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation

Eur J Immunol. 1995 Feb;25(2):418-25. doi: 10.1002/eji.1830250217.


The early thymus precursor population of adult mice has the capacity to generate T cells, B cells and dendritic cells (DC). These precursors were injected into the thymus of irradiated recipients in order to follow the kinetics of thymic DC development. The resultant cohort of T-lineage cells developing in the thymus was accompanied by a parallel cohort of DC, present at 10(3)-fold lower frequency. The intrathymic lifespan of these DC was as short as that of T-lineage thymocytes. As the thymic DC matured, some markers characteristic of the original precursor population gradually declined (Ly-5, c-kit, Sca-2) whereas markers characteristic of thymic DC appeared and were maintained (major histocompatibility complex class II, CD11c, NLDC-145 and CD8 alpha). Some thymic DC expressed the early B-cell marker BP-1, and BP-1 mRNA, throughout their maturation. The surface markers on thymic DC could be divided into two groups. Some markers, including class I and class II MHC, CD8 alpha and BP-1, appeared to be integral components of the DC surface. In contrast, other markers, including Thy-1, CD4 and CD8 beta, had probably been picked up from associated thymocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / analysis*
  • Base Sequence
  • CD8 Antigens / analysis
  • Cells, Cultured
  • DNA-Binding Proteins / analysis
  • Dendritic Cells / immunology
  • Dendritic Cells / physiology*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Nerve Tissue Proteins / analysis
  • RNA-Binding Proteins*
  • T-Lymphocytes / physiology
  • Thy-1 Antigens / analysis
  • Thymus Gland / cytology*


  • Antigens, Surface
  • CD8 Antigens
  • DNA-Binding Proteins
  • Nerve Tissue Proteins
  • RNA-Binding Proteins
  • Thy-1 Antigens