Comparison of Type IV-pilin Genes of Pseudomonas Aeruginosa of Various Habitats Has Uncovered a Novel Unusual Sequence

FEMS Microbiol Lett. 1995 Jan 15;125(2-3):265-73. doi: 10.1111/j.1574-6968.1995.tb07367.x.

Abstract

All known pilin sequences in Pseudomonas aeruginosa were amplified by a set of consensus primers located in the 5"-conserved region of pilA and the threonine-specific t-RNA following pilA. This also enabled the discovery of a novel pilin gene in a strain pair of clonal variants, which differs from known pilin genes in its increased GC-content. The mature protein has 173 amino acids making it the longest pilin known to date in P. aeruginosa. Different inserted sequences detected between the 3"-end of the pilin gene and the t-RNA in this strain and in strains with group I pilin genes seemed to be specific for each pilin group indicating a horizontal cotransfer of sequences.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Outer Membrane Proteins / ultrastructure
  • Base Sequence
  • Codon
  • Consensus Sequence
  • Conserved Sequence
  • DNA Primers
  • Fimbriae Proteins
  • Flagella / ultrastructure
  • Genes, Bacterial*
  • Microscopy, Electron
  • Molecular Sequence Data
  • Pseudomonas aeruginosa / genetics*
  • Pseudomonas aeruginosa / isolation & purification
  • Pseudomonas aeruginosa / ultrastructure
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Outer Membrane Proteins
  • Codon
  • DNA Primers
  • Fimbriae Proteins

Associated data

  • GENBANK/L37109