Spectroscopic evidence for a common electron transfer pathway for two tryptophan tryptophylquinone enzymes

J Biol Chem. 1995 Mar 3;270(9):4293-8. doi: 10.1074/jbc.270.9.4293.

Abstract

Aromatic amine dehydrogenase (AADH) and methylamine dehydrogenase (MADH) are the only two enzymes known to use the cofactor tryptophan tryptophylquinone (TTQ). Each catalyzes oxidative deamination of a distinct class of primary amines. A detailed comparison of their circular dichroic spectra indicates that both proteins share a similar fold with their TTQ cofactors residing in similar environments and that this may be a useful diagnostic probe for TTQ enzymes. Alcaligenes faecalis cells induced to express AADH also express a large amount of the blue copper protein, azurin. Oxidized azurin is rapidly reduced by a catalytic amount of AADH in the presence of the substrate, tyramine. Three A. faecalis cytochromes-c and three other cytochromes-c were tested for electron transfer activity with AADH. Azurin markedly facilitated electron transfer from AADH to each cytochrome. This suggests that AADH and azurin may form an electron transfer complex with a c-type cytochrome, analogous to the crystallographically determined MADH-amicyanin-cytochrome c-551i complex (Chen, L., Durley, R. C. E., Matthews, F. S., and Davidson, V. L. (1994) Science 264, 86-90). The similarities of MADH and AADH plus the demonstration of azurin and multiple cytochromes as functional electron-transfer partners suggest that both TTQ-bearing enzymes share common mechanisms for oxidative deamination and subsequent electron transfer.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcaligenes / enzymology
  • Azurin / metabolism
  • Circular Dichroism
  • Cytochrome c Group / metabolism
  • Electron Transport
  • Indolequinones*
  • Oxidoreductases Acting on CH-NH Group Donors / chemistry
  • Oxidoreductases Acting on CH-NH Group Donors / metabolism*
  • Quinones / metabolism*
  • Spectrophotometry, Ultraviolet
  • Tryptophan / analogs & derivatives*
  • Tryptophan / metabolism

Substances

  • Cytochrome c Group
  • Indolequinones
  • Quinones
  • Azurin
  • tryptophan tryptophylquinone
  • Tryptophan
  • methylamine dehydrogenase
  • Oxidoreductases Acting on CH-NH Group Donors
  • aromatic amine dehydrogenase