Isolation of a novel cDNA encoding a putative membrane receptor with high homology to the cloned mu, delta, and kappa opioid receptors

Brain Res Mol Brain Res. 1994 Nov;27(1):37-44. doi: 10.1016/0169-328x(94)90181-3.


A rat brain cDNA library was screened for clones homologous to the recently cloned mouse delta-opioid receptor (DOR-1). Among the clones isolated was Hyp 8-1, a clone with a unique nucleotide sequence capable of encoding a putative protein which is 57-58% identical to the amino acid sequences of the cloned delta, mu and kappa opioid receptors, indicating a close relationship of Hyp 8-1 with the opioid receptor family. Several cDNAs representing possible splice variants of Hyp 8-1 were also isolated. Binding studies of COS-7 cells transfected with clone Hyp 8-1 failed to demonstrate specific binding with several 3H-opioid ligands. In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors. Thus, it is likely that the Hyp 8-1 cDNA encodes a novel peptide receptor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain Chemistry
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary / genetics*
  • Ganglia, Spinal / chemistry*
  • Ligands
  • Male
  • Mice
  • Molecular Sequence Data
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Opioid / classification
  • Receptors, Opioid / genetics*
  • Receptors, Opioid, delta / genetics
  • Receptors, Opioid, kappa / genetics
  • Receptors, Opioid, mu / genetics
  • Sequence Alignment
  • Sequence Homology


  • DNA, Complementary
  • Hyp 8-1 protein, rat
  • Ligands
  • Receptors, Opioid
  • Receptors, Opioid, delta
  • Receptors, Opioid, kappa
  • Receptors, Opioid, mu

Associated data

  • GENBANK/L29419