Single polymerase chain reaction for the detection of tetracycline-resistant determinants Tet K and Tet L

Mol Cell Probes. 1994 Oct;8(5):417-22. doi: 10.1006/mcpr.1994.1059.

Abstract

Oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants Tet K and Tet L. Forty-three isolates representing 11 genera carrying Tet K and/or Tet L determinants gave appropriate PCR products. The PCR products hybridized with labelled Tet K or Tet L probes, and differentiated the Tet K from the Tet L PCR products. We confirmed that two Haemophilus aphrophilus carried the Tet K determinant and two Veillonella parvula carried the Tet L determinant. This is the first time that either of these two genes have been found in Gram-negative species. Four vaginal samples, previously positive with the Tet M/O PCR assay, were also positive with the Tet K/L assay. This is the first report of a PCR assay for the detection of Tet K and Tet L determinants in bacteria or clinical specimens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Evaluation Studies as Topic
  • Female
  • Genes, Bacterial*
  • Haemophilus / genetics
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • Staphylococcal Infections / microbiology
  • Tetracycline Resistance / genetics*
  • Vaginosis, Bacterial / microbiology
  • Veillonella / genetics

Substances

  • DNA Primers

Associated data

  • GENBANK/J01764
  • GENBANK/J01765
  • GENBANK/M16217
  • GENBANK/X08034