Possible proton relay pathways in cytochrome c oxidase

Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1604-8. doi: 10.1073/pnas.92.5.1604.


As the final electron acceptor in the respiratory chain of eukaryotic and many prokaryotic organisms, cytochrome c oxidase (EC catalyzes the reduction of oxygen to water and generates a proton gradient. To test for proton pathways through the oxidase, site-directed mutagenesis was applied to subunit I of the Rhodobacter sphaeroides enzyme. Mutants were characterized in three highly conserved regions of the peptide, comprising possible proton loading, unloading, and transfer sites: an interior loop between helices II and III (Asp132Asn/Ala), an exterior loop between helices IX and X (His411Ala, Asp412Asn, Thr413Asn, Tyr414Phe), and the predicted transmembrane helix VIII (Thr352Ala, Pro358Ala, Thr359Ala, Lys362Met). Most of the mutants had lower activity than wild type, but only mutants at residue 132 lost proton pumping while retaining electron transfer activity. Although electron transfer was substantially inhibited, no major structural alteration appears to have occurred in D132 mutants, since resonance Raman and visible absorbance spectra were normal. However, lower CO binding (70-85% of wild type) suggests some minor change to the binuclear center. In addition, the activity of the reconstituted Asp132 mutants was inhibited rather than stimulated by ionophores or uncoupler. The inhibition was not observed with the purified enzyme and a direct pH effect was ruled out, suggesting an altered response to the electrical or pH gradient. The results support an important role for the conserved II-III loop in the proton pumping process and are consistent with the possibility of involvement of residues in helix VIII and the IX-X loop.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
  • Electron Transport
  • Electron Transport Complex IV / chemistry
  • Electron Transport Complex IV / metabolism*
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Proton-Translocating ATPases / metabolism*
  • Rhodobacter sphaeroides / enzymology
  • Spectrum Analysis, Raman
  • Structure-Activity Relationship
  • Valinomycin / pharmacology


  • Bacterial Proteins
  • Valinomycin
  • Carbonyl Cyanide m-Chlorophenyl Hydrazone
  • Electron Transport Complex IV
  • Proton-Translocating ATPases