Abstract
Pineal opsin (P-opsin), an opsin from chick that is highly expressed in pineal but is not detectable in retina, was cloned by the polymerase chain reaction. It is likely that the P-opsin lineage diverged from the retinal opsins early in opsin evolution. The amino acid sequence of P-opsin is 42 to 46 percent identical to that of the retinal opsins. P-opsin is a seven-membrane spanning, G protein-linked receptor with a Schiff-base lysine in the seventh membrane span and a Schiff-base counterion in the third membrane span. The primary sequence of P-opsin suggests that it will be maximally sensitive to approximately 500-nanometer light and produce a slow and prolonged phototransduction response consistent with the nonvisual function of pineal photoreception.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Avian Proteins
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Base Sequence
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Biological Evolution
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Brain Chemistry
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Chickens
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Cloning, Molecular
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Molecular Sequence Data
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / chemistry*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / physiology
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Pineal Gland / chemistry*
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Protein Structure, Secondary
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RNA, Messenger / analysis
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Retina / chemistry
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Rod Opsins / analysis
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Rod Opsins / chemistry*
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Rod Opsins / genetics
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Rod Opsins / physiology
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Sequence Homology, Amino Acid
Substances
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Avian Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Rod Opsins
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pinopsin protein, chicken
Associated data
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GENBANK/S75717
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GENBANK/S75718
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GENBANK/S75719
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GENBANK/S75720
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GENBANK/S75785