Abstract
Transduction of DBMC with the neo gene did not negatively affect the viability of DBMC or the graft-prolonging effect of DBMC infusion and provided a sensitive method to monitor DBMC-derived chimerism at a microchimeric level for at least 6 months by molecular and immunohistochemical techniques. Selection of neo-transduced DBMC with G418 prior to infusion will enable more uniform neo expression and will provide novel opportunities to further investigate chimerism in primates.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Bone Marrow Cells
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Bone Marrow Transplantation / physiology*
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Chimera*
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DNA Primers
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Gene Transfer Techniques*
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Genes, Bacterial
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Genetic Markers
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Graft Survival
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Immunohistochemistry
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Kidney Transplantation / physiology*
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Macaca mulatta
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Male
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Molecular Sequence Data
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Oligonucleotide Probes
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Time Factors
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Transplantation, Homologous
Substances
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DNA Primers
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Genetic Markers
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Oligonucleotide Probes