A gene transfer method for the study of chimerism derived from donor bone marrow in organ transplant recipients

Transplant Proc. 1995 Feb;27(1):158-9.

Abstract

Transduction of DBMC with the neo gene did not negatively affect the viability of DBMC or the graft-prolonging effect of DBMC infusion and provided a sensitive method to monitor DBMC-derived chimerism at a microchimeric level for at least 6 months by molecular and immunohistochemical techniques. Selection of neo-transduced DBMC with G418 prior to infusion will enable more uniform neo expression and will provide novel opportunities to further investigate chimerism in primates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow Cells
  • Bone Marrow Transplantation / physiology*
  • Chimera*
  • DNA Primers
  • Gene Transfer Techniques*
  • Genes, Bacterial
  • Genetic Markers
  • Graft Survival
  • Immunohistochemistry
  • Kidney Transplantation / physiology*
  • Macaca mulatta
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Time Factors
  • Transplantation, Homologous

Substances

  • DNA Primers
  • Genetic Markers
  • Oligonucleotide Probes