Excessive function of peripheral blood neutrophils from patients with Behçet's disease and from HLA-B51 transgenic mice

Arthritis Rheum. 1995 Mar;38(3):426-33. doi: 10.1002/art.1780380321.


Objective: To elucidate the role played by HLA-B51 in the neutrophil hyperfunction of Behçet's disease, we determined the superoxide production by purified peripheral blood neutrophils from Behçet's disease patients, from HLA-B51 positive healthy individuals, and from HLA-B51 transgenic mice.

Methods: Neutrophil function was evaluated by flow cytometric analysis, detecting the conversion of 2',7'-dichlorofluorescin diacetate into dichloroflurescein, induced by superoxide in the neutrophils.

Results: A significant correlation between the neutrophil hyperfunction and the possession of HLA-B51 phenotype, regardless of the presence of the disease, was observed in humans. FMLP-stimulated neutrophils (without in vitro priming) from HLA-B51 transgenic mice, but not those from HLA-B35 transgenic mice or from nontransgenic mice, produced substantial amounts of superoxide.

Conclusion: The HLA-B51 molecule itself may be responsible, at least in part, for neutrophil hyperfunction in Behçet's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behcet Syndrome / immunology*
  • Female
  • Flow Cytometry
  • HLA-B Antigens* / analysis
  • HLA-B Antigens* / physiology
  • HLA-B51 Antigen
  • Humans
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Transgenic
  • Neutrophils / immunology
  • Neutrophils / physiology*
  • Phenotype
  • Superoxides / blood*


  • HLA-B Antigens
  • HLA-B51 Antigen
  • Superoxides