Murine M290 integrin expression modulated by mast cell activation

Immunity. 1994 Aug;1(5):393-403. doi: 10.1016/1074-7613(94)90070-1.


Intraepithelial T lymphocytes possess a (TGF beta)-inducible integrin complex that consists of the beta 7 integrin chain in association with a novel alpha chain, murine alpha M290. Using antibodies directed against the murine alpha M290 chain, IL-3-dependent murine bone marrow-derived mast cells demonstrated positive staining after induction with TGF beta. Sequence information from the human intraepithelial T lymphocyte-alpha E gene sequence was used to clone the murine homolog from a TGF beta-induced T cell library. The murine alpha E homolog gene encodes a protein recognized by antibodies to alpha M290. Expression of this gene by bone marrow-derived mast cells was found to be induced by TGF beta or IgE-mediated cross-linking of Fc epsilon R1. Furthermore, exposure of murine TK-1 cells to supernatants from activated mast cells induces the expression of the alpha M290 gene, indicating that a mast cell mediator(s) can act in a paracrine manner to induce expression of this integrin complex by other cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Degranulation
  • Gene Expression
  • Humans
  • Immunoglobulin E / pharmacology
  • Integrins / chemistry*
  • Integrins / genetics*
  • Mast Cells / immunology
  • Mast Cells / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Structure
  • Proteins / analysis


  • Integrins
  • Proteins
  • Immunoglobulin E

Associated data

  • GENBANK/U12236