Complementary DNAs for two arylamine N-acetyltransferases with identical 5' non-coding regions from rat pineal gland

Eur J Biochem. 1995 Feb 15;228(1):129-37. doi: 10.1111/j.1432-1033.1995.tb20240.x.


A cDNA library was constructed from the pineal gland of rats injected with isoproterenol and screened with 32P-labeled cDNAs encoding arylamine N-acetyltransferases from rabbit and human liver. Two types of cDNAs for arylamine N-acetyltransferases (A-type and B-type) were isolated. Expression of the cDNAs in Chinese hamster ovary cells indicated that A-type N-acetyltransferase acetylates both arylamines and arylalkylamines, while the B-type enzyme acetylates only arylamines. Therefore, neither the A-type nor the B-type of enzyme seems to be the arylalkylamine N-acetyltransferase involved in melatonin synthesis in the pineal gland. Nucleotide sequence analysis revealed that both A-type and B-type cDNAs code for 290 amino acids, and that they showed 82.8% similarity in the coding region. However, the nucleotide sequence in the 5' non-coding region was identical in the A-type and B-type cDNAs. In addition, the 5' non-coding region contained another possible open reading frame for 79 amino acids. Data base research revealed that the complementary sequence of the 5' non-coding region has high similarity with the coding regions of cDNAs for high-mobility-group proteins (HMG) 1 and 2, which are thought to regulate mRNA transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arylamine N-Acetyltransferase / chemistry
  • Arylamine N-Acetyltransferase / genetics*
  • Base Sequence
  • Blotting, Southern
  • DNA, Complementary / chemistry
  • DNA, Complementary / isolation & purification*
  • High Mobility Group Proteins / genetics
  • Molecular Sequence Data
  • Pineal Gland / enzymology*
  • Rats
  • Rats, Wistar


  • DNA, Complementary
  • High Mobility Group Proteins
  • Arylamine N-Acetyltransferase

Associated data

  • GENBANK/U01343
  • GENBANK/U01344
  • GENBANK/U01345
  • GENBANK/U01346
  • GENBANK/U01347
  • GENBANK/U01348