Abstract
The nucleotide (nt) sequence of the Helicobacter pylori (Hp) napA gene, encoding neutrophil-activating protein A (HPNAP) was determined. Alignment of this sequence with those of known bacterioferritins (Bfr) revealed sequence homology and conservation of a 7-amino-acid (aa) motif constituting the ferroxidase (Frx) center of Bfr in the HPNAP. The N-terminal aa sequence deduced from the iron-regulated mrgC gene of Bacillus subtilis [Chen et al., J. Bacteriol. 175 (1993) 5428-5437] is highly similar to that of HPNAP and contains five Frx center aa residues. The deduced aa sequences for proteins of unknown function in Treponema pallidum [Walfield et al., Infect. Immun. 57 (1989) 633-635] and in the cyanobacterium Anabaena variabilis [Sato, GenBank accession No. JU0384 (1991)] identify these two proteins as Bfr. Although the DNA-binding protein from starved cells of Escherichia coli [Almiron et al., Genes Dev. 6 (1992) 2646-2654] is clearly a HPNAP/Bfr homologue, a significant part of its Frx center is missing. It is unlikely that the intracellular function of HPNAP is related to its ability to activate neutrophils.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Anabaena / chemistry
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Anabaena / genetics*
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Bacillus subtilis / chemistry
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Bacillus subtilis / genetics*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Consensus Sequence
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Cytochrome b Group / genetics
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Cytochrome b Group / isolation & purification*
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Cytochromes / genetics
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DNA-Binding Proteins / genetics
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Ferritins / genetics
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Ferritins / isolation & purification*
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Genes, Bacterial
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Genes, Plant
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Genes, Protozoan
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Helicobacter pylori / chemistry
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Helicobacter pylori / genetics*
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Iron / metabolism
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Molecular Sequence Data
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Plant Proteins / genetics
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Plant Proteins / isolation & purification*
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Protozoan Proteins / genetics
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Protozoan Proteins / isolation & purification*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Treponema pallidum / chemistry
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Treponema pallidum / genetics*
Substances
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Bacterial Proteins
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Cytochrome b Group
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Cytochromes
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DNA-Binding Proteins
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Plant Proteins
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Protozoan Proteins
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neutrophil-activating protein A, Helicobacter pylori
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Ferritins
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bacterioferritin
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Iron