A Phosphate-Repressible, High-Affinity Phosphate Permease Is Encoded by the pho-5+ Gene of Neurospora Crassa

Gene. 1995 Feb 3;153(1):135-9. doi: 10.1016/0378-1119(94)00814-9.

Abstract

The pho-5+ gene of Neurospora crassa, which encodes a high-affinity phosphate permease, has been cloned and analyzed. The deduced ORF of 1707 nucleotides is interrupted by a single 63-nt intron and codes for a protein of 569 amino acids (aa). This aa sequence has 48% identity with the high-affinity phosphate transporter of Saccharomyces cerevisiae, PHO84. The pho-5 null mutants have no obvious phenotype. Strains which contain a null mutation in pho-4, which encodes an additional high-affinity phosphate permease [Bowman et al., J. Bacteriol. 153 (1983) 292-296], also have no obvious phenotype. However, strains containing mutations in both pho-5 and pho-4 are unable to grow under phosphate-restrictive conditions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Enzyme Induction
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism
  • Molecular Sequence Data
  • Mutation
  • Neurospora crassa / enzymology
  • Neurospora crassa / genetics*
  • Neurospora crassa / growth & development
  • Phosphate Transport Proteins*
  • Phosphates / metabolism

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Isoenzymes
  • Membrane Transport Proteins
  • Phosphate Transport Proteins
  • Phosphates
  • phosphate permease

Associated data

  • GENBANK/L36127