Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice

Mech Ageing Dev. 1994 Oct 20;76(2-3):215-24. doi: 10.1016/0047-6374(94)91595-4.

Abstract

The objective of this study was to explore the role of molecular oxidative damage and caloric intake in the aging process. The concentration of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was compared in five different tissues of mice (skeletal muscle, brain, heart, liver and kidney) as a function of age and in response to dietary restriction. A comparison of 8- and 27-month-old mice indicated that the age-related increase in 8-OHdG concentration was greater in skeletal muscle, brain and heart, which are primarily composed of long-lived, post-mitotic cells, than in liver and kidney, which consist of slow-dividing cells. Dietary restricted (DR) mice kept on 60% caloric intake as compared to the ad libitum-fed (AL) mice showed a lower concentration in 8-OHdG content in all the tissues compared to AL mice. The DR-related amelioration of DNA oxidative damage was greater in the post-mitotic tissues compared to those undergoing slow mitoses. Results support the hypothesis that oxidative damage to long-lived post-mitotic cells may be a key factor in the aging process.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aging / metabolism*
  • Animals
  • Brain / metabolism
  • DNA / metabolism
  • DNA Damage*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Energy Intake
  • Food Deprivation / physiology*
  • Kidney / metabolism
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muscle, Skeletal / metabolism
  • Myocardium / metabolism
  • Oxidative Stress
  • Rats
  • Rats, Sprague-Dawley
  • Tissue Distribution

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • DNA
  • Deoxyguanosine