Polycyclic Aromatic hydrocarbon-DNA and Protein Adducts in Coal Tar Treated Patients and Controls and Their Relationship to Glutathione S-transferase Genotype

Mutat Res. 1995 Apr;334(2):117-24. doi: 10.1016/0165-1161(95)90001-2.

Abstract

Coal tar treated psoriasis patients were used as a model population to evaluate a panel of immunoassays for monitoring exposure to benzo[a]pyrene (BP) and related polycyclic aromatic hydrocarbons (PAH). The assays included measurement of PAH diol epoxide-DNA adducts in white blood cells by competitive enzyme-linked immunosorbent assay (ELISA) with fluorescence endpoint detection, PAH-albumin adducts by competitive ELISA with color endpoint detection and serum levels of antibodies recognizing BP diol epoxide-DNA adducts by noncompetitive color ELISA. PAH-DNA adducts by ELISA were elevated in patients (mean 6.77 +/- 12.05/10(8)) compared to controls (4.90 +/- 8.81/10(8), p = 0.12). There was no difference in PAH-albumin adducts between patients (mean 0.61 +/- 0.31 fmol/micrograms) and controls (0.63 +/- 0.30 fmol/micrograms). Glutathione S-transferase M1 genotype was also determined but no relationship was found between presence of the gene and either DNA or protein adduct levels. About 30% of both patients and controls had measurable titer of antibodies recognizing BPDE-I-DNA adducts. Measurement of white blood cell DNA adducts by ELISA was the most sensitive method for detecting PAH exposure in coal tar-treated psoriasis patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Benzo(a)pyrene / metabolism*
  • Biomarkers
  • Carcinogens / metabolism*
  • Case-Control Studies
  • Coal Tar / adverse effects
  • Coal Tar / metabolism*
  • DNA Adducts / biosynthesis*
  • Environmental Exposure / adverse effects
  • Environmental Monitoring / methods
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • Genotype
  • Glutathione Transferase / genetics*
  • Glutathione Transferase / metabolism
  • Humans
  • Immunoassay
  • Inactivation, Metabolic
  • Linear Models
  • Male
  • Middle Aged
  • Polycyclic Compounds / metabolism
  • Protein Binding
  • Psoriasis / drug therapy
  • Serum Albumin / metabolism
  • Skin / metabolism
  • Smoking

Substances

  • Biomarkers
  • Carcinogens
  • DNA Adducts
  • Polycyclic Compounds
  • Serum Albumin
  • Benzo(a)pyrene
  • Coal Tar
  • Glutathione Transferase