A broadly neutralizing human monoclonal antibody against gp41 of human immunodeficiency virus type 1

AIDS Res Hum Retroviruses. 1994 Dec;10(12):1651-8. doi: 10.1089/aid.1994.10.1651.

Abstract

We have established a hybridoma clone, designated 2F5, secreting a neutralizing human monoclonal antibody (MAb) specific for gp41 of human immunodeficiency virus type 1 (HIV-1). The epitope of MAb 2F5 was mapped to amino acid sequence Glu-Leu-Asp-Lys-Trp-Ala on the ectodomain of gp41. In this study different in vitro test systems were used to characterize the neutralizing properties of MAb 2F5. In syncytium inhibition assays, fusion inhibition experiments, and neutralization assays on different HIV-susceptible cells (H9, U937, and peripheral blood mononuclear cells) MAb 2F5 showed broad-spectrum neutralizing capacity against HIV-1 laboratory isolates IIIB, MN, RF, and SF2. In addition, primary isolates from AIDS patients were also neutralized.

MeSH terms

  • Acquired Immunodeficiency Syndrome / immunology
  • Amino Acid Sequence
  • Antibodies, Monoclonal / immunology*
  • Cell Fusion
  • Cell Line
  • Epitope Mapping
  • Giant Cells
  • HIV Antibodies / immunology*
  • HIV Core Protein p24 / immunology
  • HIV Envelope Protein gp41 / immunology*
  • HIV-1 / immunology*
  • HIV-1 / isolation & purification
  • Humans
  • Hybridomas
  • Immunodominant Epitopes / immunology
  • Molecular Sequence Data
  • Neutralization Tests

Substances

  • Antibodies, Monoclonal
  • HIV Antibodies
  • HIV Core Protein p24
  • HIV Envelope Protein gp41
  • Immunodominant Epitopes