Solid phase extraction and high performance liquid chromatographic determination of dobutamine in plasma of dialysed patients

Biomed Chromatogr. 1994 Nov-Dec;8(6):309-12. doi: 10.1002/bmc.1130080613.


An isocratic reversed-phase high performance liquid chromatographic method has been developed for th e determination of dobutamine in the plasma of dialysed patients. A solid phase extraction method with a Sep-Pak C18 cartridge was used to isolate the drug and isoxsuprine (internal standard) from plasma. The separation was carried out on an ODS-Hypersil column with 0.1 M phosphate buffer:acetonitrile:methanol (72:20:8 v/v/v) as the mobile phase. The recovery of dobutamine added to plasma by the extraction procedure was 87 +/- 2.3% (mean +/- SD). The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0-1000 ng/mL. Quantification was by fluorescence detection at 275 nm excitation and 310 nm emission wavelengths with a detection limit of 5 ng/mL for dobutamine. This procedure was applied to ascertain the pharmacokinetics of dobutamine infusion in nine patients with cardiogenic shock and end-stage renal disease undergoing haemodialysis.

MeSH terms

  • Chemical Phenomena
  • Chemistry, Physical
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, High Pressure Liquid / statistics & numerical data
  • Dobutamine / blood*
  • Dobutamine / chemistry
  • Dobutamine / pharmacokinetics
  • Humans
  • Renal Dialysis*
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Dobutamine