Induction of NF-AT in normal B lymphocytes by anti-immunoglobulin or CD40 ligand in conjunction with IL-4

Immunity. 1994 Jun;1(3):179-87. doi: 10.1016/1074-7613(94)90096-5.


We show here that ligation of surface immunoglobulin or CD40 receptors in conjunction with interleukin-4 induces the nuclear factor of activated T cells (NF-AT) in normal murine B cells, which is inhibited by cyclosporin (CsA). Lipopolysaccharide, which activates B cells by a Ca(2+)-independent, CsA-resistant pathway, does not induce NF-AT. The NF-AT complex in T cells and B cells appears to be identical, comprising both Fos and Jun proteins and the 120 kDa cytosolic component of NF-AT (NF-ATp). Our transfection experiments using a trimerized NF-AT site linked to the minimal IL-2 promoter driving luciferase activity demonstrate that NF-AT is functional in A20 B-lymphoma cells. These results therefore suggest that the induction of NF-AT forms part of the B cell response to both cross-linking antigens and T cell-generated signals.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / pharmacology
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Base Sequence
  • CD40 Ligand
  • Cell Line
  • Cross-Linking Reagents
  • Cyclosporine / pharmacology
  • DNA / genetics
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • Enhancer Elements, Genetic
  • In Vitro Techniques
  • Interleukin-2 / genetics
  • Interleukin-4 / pharmacology
  • Male
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Molecular Sequence Data
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Proto-Oncogene Proteins c-fos / metabolism
  • Proto-Oncogene Proteins c-jun / metabolism
  • T-Lymphocytes / immunology
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics
  • Transfection


  • Antibodies, Anti-Idiotypic
  • Cross-Linking Reagents
  • DNA-Binding Proteins
  • Interleukin-2
  • Membrane Glycoproteins
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Transcription Factors
  • CD40 Ligand
  • Interleukin-4
  • Cyclosporine
  • DNA