Overexpression of Matrix metalloproteinase-7 mRNA in Human Colon Carcinomas

Cancer. 1995 Mar 15;75(6 Suppl):1516-9. doi: 10.1002/1097-0142(19950315)75:6+<1516::aid-cncr2820751522>3.0.co;2-7.

Abstract

Background: Matrix metalloproteinase-7 (MMP-7) is a member of the family of matrix-degrading metalloproteinases (MMPs) that are considered to contribute to cancer invasion and metastasis. One of the gene products that the authors have isolated by a cDNA subtraction of human gastric cancer minus normal gastric mucosa is MMP-7. The purpose of this study was to examine the expression of MMP-7 mRNA in different stages of human colorectal carcinomas.

Methods: A subtracted complementary DNA library was generated from a paired sample of human gastric carcinoma minus normal gastric mucosa. Screening of the library clones determined that MMP-7 mRNA was overexpressed. The complementary DNA insert was then used as a probe to evaluate the expression in a larger number of surgical samples of human colorectal carcinomas by Northern hybridization.

Results: The mRNA signal was greater in the colorectal carcinoma than in paired adjacent normal colonic or rectal mucosa in 39 of 47 cases. The expression of MMP-7 mRNA in tumor tissues increased with increasing Dukes' stage (P < 0.05). The MMP-7 mRNA expression was greatest in the metastatic liver lesions.

Conclusions: The findings imply that MMP-7 mRNA is overexpressed in human colorectal carcinomas and that MMP-7 may prove useful as a marker of biologic aggressiveness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology
  • Adenocarcinoma / genetics
  • Adult
  • Aged
  • Biomarkers, Tumor
  • Blotting, Northern
  • Colonic Neoplasms / enzymology*
  • Colonic Neoplasms / genetics
  • Female
  • Genomic Library
  • Humans
  • Male
  • Matrix Metalloproteinase 7
  • Metalloendopeptidases / biosynthesis*
  • Middle Aged
  • RNA, Messenger / analysis

Substances

  • Biomarkers, Tumor
  • RNA, Messenger
  • Metalloendopeptidases
  • Matrix Metalloproteinase 7