Molecular cloning and functional expression of a novel potassium channel beta-subunit from human atrium

FEBS Lett. 1995 Mar 13;361(1):13-6. doi: 10.1016/0014-5793(95)00120-x.

Abstract

We report the cloning and functional expression of a novel K+ channel beta-subunit from human atrium, hKv beta 3. hKv beta 3 is highly homologous to the two beta-subunits cloned from rat brain, Kv beta 1 and Kv beta 2, but has an essentially unique stretch of 79 N-terminal residues. Upon expression in Xenopus oocytes, hKv beta 3 accelerates the inactivation of co-injected hKv1.4 currents and induces fast inactivation of non-inactivating co-injected hKv1.5 currents. By contrast, hKv beta 3 had no effect on hKv1.1, hKv1.2, or hKv2.1 currents. Thus, hKv beta 3 represents a third type of K+ channel beta-subunit which modulates the kinetics of a unique subset of channels in the Kv1 subfamily.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Atrial Function*
  • Base Sequence
  • Cloning, Molecular
  • Humans
  • Ion Channel Gating / genetics*
  • Membrane Potentials
  • Molecular Sequence Data
  • Oocytes
  • Potassium Channels / biosynthesis
  • Potassium Channels / genetics*
  • Potassium Channels / physiology*
  • RNA, Messenger / genetics
  • Rats
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Xenopus

Substances

  • Potassium Channels
  • RNA, Messenger

Associated data

  • GENBANK/U16953