Wortmannin inhibits insulin-stimulated but not contraction-stimulated glucose transport activity in skeletal muscle

FEBS Lett. 1995 Mar 13;361(1):51-4. doi: 10.1016/0014-5793(95)00147-2.


In skeletal muscle, glucose transport is stimulated by insulin, contractions and hypoxia. In this study, we used the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin to examine whether (i) PI 3-kinase activity is necessary for stimulation of glucose transport by insulin in muscle, and (ii) PI 3-kinase mediates a step in the pathway by which contractions/hypoxia stimulate glucose transport. Wortmannin completely blocked insulin- and insulin-like growth factor-1-stimulated glucose transport in muscle. In contrast, wortmannin had no effect on the stimulation of glucose transport by contractions or hypoxia, providing evidence that PI 3-kinase activity is not involved in the activation of glucose transport by these stimuli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androstadienes / pharmacology*
  • Animals
  • Biological Transport / drug effects
  • Cell Hypoxia
  • Culture Techniques
  • Glucose / metabolism*
  • Insulin / pharmacology
  • Insulin-Like Growth Factor I / pharmacology
  • Male
  • Muscle Contraction / physiology
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / enzymology*
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor) / antagonists & inhibitors
  • Phosphotransferases (Alcohol Group Acceptor) / physiology*
  • Rats
  • Rats, Wistar
  • Wortmannin


  • Androstadienes
  • Insulin
  • Insulin-Like Growth Factor I
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Glucose
  • Wortmannin