Localization of basic fibroblast growth factor in bovine adrenal chromaffin cells

J Neurochem. 1995 Apr;64(4):1521-7. doi: 10.1046/j.1471-4159.1995.64041521.x.

Abstract

We have investigated basic fibroblast growth factor (FGF-2) localization in and release from isolated bovine adrenal chromaffin cells. In contrast to previous reports, we found no evidence of fibroblast growth factor (FGF) storage in catecholamine-containing chromaffin granules. Subcellular fractionation studies did not show enrichment of FGF-2 immunoreactivity in granules, and cholinergic stimulation failed to release FGF-2 into the medium. Our results suggest that adrenal chromaffin cells resemble other FGF-2-synthesizing cell types with respect to FGF storage and secretion.

MeSH terms

  • Adrenal Medulla / cytology
  • Adrenal Medulla / metabolism*
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Cattle
  • Chromaffin System / cytology
  • Chromaffin System / metabolism*
  • Fibroblast Growth Factor 2 / metabolism*
  • Immunohistochemistry
  • Microscopy, Immunoelectron
  • Stimulation, Chemical
  • Subcellular Fractions / metabolism
  • Tissue Distribution

Substances

  • Fibroblast Growth Factor 2