Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene

Mol Microbiol. 1994 Nov;14(4):809-22. doi: 10.1111/j.1365-2958.1994.tb01317.x.


Legionella pneumophila dot mutations cause defects in intracellular targeting of the microorganism within cultured macrophages. Each of the previously characterized dot mutations was shown to be complemented by a single open reading frame designated dotA. The defects caused by the mutations appear to be due to disrupted function of the predicted 1048-amino-acid residue DotA protein, and not by polarity effects on a downstream gene. Complementation studies indicated that the product of the dotA53 mutation results in a partially functional DotA protein, consistent with a stable N-terminal fragment having biological activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Cell Division / genetics
  • Cell Line
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Genetic Linkage
  • Humans
  • Legionella pneumophila / genetics*
  • Legionella pneumophila / growth & development*
  • Legionella pneumophila / ultrastructure
  • Macrophages / microbiology
  • Microscopy, Electron
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutation*
  • Open Reading Frames


  • Bacterial Proteins
  • DNA Primers
  • DNA, Bacterial

Associated data

  • GENBANK/U07940