Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Mar 14;92(6):2224-8.
doi: 10.1073/pnas.92.6.2224.

The Origin Recognition Complex Interacts With a Bipartite DNA Binding Site Within Yeast Replicators

Free PMC article

The Origin Recognition Complex Interacts With a Bipartite DNA Binding Site Within Yeast Replicators

H Rao et al. Proc Natl Acad Sci U S A. .
Free PMC article


Replicators are genetically defined elements within chromosomes that determine the location of origins of DNA replication. In the yeast Saccharomyces cerevisiae, the ARS1 replicator contains multiple functional DNA elements: an essential A element and three important B elements--B1, B2, and B3. Functionally similar A, B1, and B2 elements are also present in the ARS307 replicator. The B3 element binds a replication and transcription enhancer protein Abf1p, whereas the A element is required for binding the origin recognition complex (ORC). The function of the B1 and B2 elements remains to be defined. We have used a gel-based DNA binding assay to study the interaction between replicators and the putative initiator protein ORC. In addition to the established requirements for ATP and the A element for ORC-DNA interaction, the new data demonstrate that sequences in the B1 element are also important for ORC-DNA association. This conclusion is supported by DNase I footprint analyses and demonstrates that ORC binds to a bipartitite recognition element within the DNA. Furthermore, mutation of nucleotides in the B1 element suggests that this element has other functions in the initiation of DNA replication besides participating in the ORC-DNA interaction.

Similar articles

See all similar articles

Cited by 95 articles

See all "Cited by" articles


    1. Nucleic Acids Res. 1991 Nov 25;19(22):6255-62 - PubMed
    1. Nature. 1992 May 14;357(6374):128-34 - PubMed
    1. Nature. 1992 May 14;357(6374):169-72 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11156-60 - PubMed
    1. Nucleic Acids Res. 1993 Feb 11;21(3):555-60 - PubMed

Publication types

MeSH terms

LinkOut - more resources