Testis and somatic Xrcc-1 DNA repair gene expression

Somat Cell Mol Genet. 1994 Nov;20(6):451-61. doi: 10.1007/BF02255837.


The human XRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose of this study was to characterize the expression of Xrcc-1 to determine if there is tissue-specific expression and to provide a baseline of information for future studies that may involve altering Xrcc-1 expression in mice. Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissues, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicate Xrcc-1 may have an important role in male germ cell physiology.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • DNA Repair / genetics*
  • Gene Expression
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Organ Specificity
  • Spermatids / ultrastructure
  • Spermatocytes / ultrastructure
  • Testis / metabolism*