Nucleotide sequence of a gene for nitrite reductase from Arabidopsis thaliana

DNA Seq. 1994;5(1):57-61. doi: 10.3109/10425179409039705.

Abstract

A nitrite reductase (NiR) gene was recovered from Arabidopsis thaliana genomic library by the homology with a cDNA of spinach NiR and sequenced. Based on the comparison with the spinach cDNA, the Arabidopsis NiR gene was concluded to contain 4 exons [exon 1 of 376 bp (beginning with ATG start codon), exon 2 of 355 bp, exon 3 of 289 bp and exon 4 of 741 bp (ending at TGA stop codon)] and 3 introns (intron 1 of 196 bp, intron 2 of 81 bp and intron 3 of 77 bp). This conclusion was confirmed by the analysis using the RT-PCR method. The deduced amino acid sequence of the coding region of the Arabidopsis NiR gene had high similarities with those of NiR genes of other plants including spinach.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Base Sequence
  • DNA, Complementary
  • DNA, Plant
  • Exons
  • Genes, Plant
  • Genomic Library
  • Introns
  • Molecular Sequence Data
  • Nitrite Reductases / genetics*

Substances

  • DNA, Complementary
  • DNA, Plant
  • Nitrite Reductases

Associated data

  • GENBANK/D14824