Polyclonals to beta-amyloid(1-42) identify most plaque and vascular deposits in Alzheimer cortex, but not striatum

Brain Res. 1994 Dec 19;667(1):138-42. doi: 10.1016/0006-8993(94)91725-6.

Abstract

Alzheimer's beta-peptides (A beta) aggregation rates depend on A beta length. We made synthetic peptide antisera to A beta 34-40 and 37-42. Purified anti-34-40 preferentially recognizes beta 1-40, vascular amyloid and a subset of plaques while purified anti-37-42 recognizes A beta 1-42 and not A beta 1-40 in dot and Western blots and immunoprecipitates; 37-42 precipitates a small percentage of fibroblast secreted A beta and strongly stains all deposits identified by monoclonals to A beta on adjacent sections from frontal cortex, but not in dorsal striatum.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alzheimer Disease / pathology*
  • Amyloid beta-Peptides / analysis*
  • Animals
  • Antibodies
  • Blotting, Western
  • Cricetinae
  • Frontal Lobe / pathology*
  • Humans
  • Immunohistochemistry
  • Neostriatum / pathology*

Substances

  • Amyloid beta-Peptides
  • Antibodies