Aflatoxin M1 in human breast milk samples from Victoria, Australia and Thailand

Food Chem Toxicol. 1995 Mar;33(3):173-9. doi: 10.1016/0278-6915(94)00130-g.


This study examined the exposure of infants to aflatoxin M1 (AFM1) and of lactating mothers to aflatoxin B1 (AFB1), using AFM1 in breast milk as a biomarker for exposure to AFB1. An enzyme-linked immunosorbent assay was modified for the analysis of AFM1 in breast milk samples from 73 women from Victoria for comparison with breast milk samples from Thailand (n = 11). The results were compared with those obtained by HPLC. AFM1 was detected in 11 samples from Victoria and five samples from Thailand at median concentrations of 0.071 ng/ml (range 0.028 to 1.031 ng/ml) and 0.664 ng/ml (range 0.039 to 1.736 ng/ml), respectively. Levels of AFM1 in Thai milk samples were significantly higher than those in milk samples from Victoria.

Publication types

  • Comparative Study

MeSH terms

  • Aflatoxin B1 / adverse effects*
  • Aflatoxin B1 / metabolism
  • Aflatoxin M1 / analysis*
  • Aflatoxin M1 / metabolism
  • Binding, Competitive
  • Biomarkers / analysis
  • Chromatography, High Pressure Liquid
  • Environmental Exposure*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Lactation
  • Linear Models
  • Milk, Human / chemistry*
  • Milk, Human / metabolism
  • Ovalbumin / metabolism
  • Pilot Projects
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Thailand
  • Victoria


  • Biomarkers
  • Aflatoxin M1
  • Ovalbumin
  • Aflatoxin B1