Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 Sep;138(1-2):85-90.

Poly(ADP-ribose) Polymerase Activity in Intact or Permeabilized Leukocytes From Mammalian Species of Different Longevity

Affiliations
  • PMID: 7898480
Comparative Study

Poly(ADP-ribose) Polymerase Activity in Intact or Permeabilized Leukocytes From Mammalian Species of Different Longevity

A Bürkle et al. Mol Cell Biochem. .

Abstract

Poly(ADP-ribosyl)ation is a eukaryotic posttranslational protein modification catalyzed by poly(ADP-ribose) polymerase (PARP), a highly conserved nuclear enzyme which uses NAD as substrate. We have previously tested PARP activity in permeabilized mononuclear blood cells (MNC) from 13 mammalian species as a function of the species-specific life span. A direct and maximal stimulus of PARP activation was provided by including saturating amounts of a double-stranded oligonucleotide in the PARP-reaction buffer. The data yielded a strong positive correlation between PARP activities and the species' maximal life spans (r = 0.84; p << 0.001). Here, we investigated the formation of poly(ADP-ribose) in living MNC from two mammalian species with widely differing longevity (rat and man) by immunofluorescence detection of poly(ADP-ribose). The fraction of positive cells was recorded, following gamma-irradiation of intact MNC, as a semiquantitative estimation of poly(ADP-ribose) formation. Human samples displayed a significantly higher percentage of positivity than did those from rats, consistent with our previous results on permeabilized cells. While rat MNC had a higher NAD content than human MNC, the number of radiation-induced DNA strand breaks was not significantly different in the two species. Since poly(ADP-ribosyl)ation is apparently involved in DNA repair and the cellular recovery from DNA damage, we speculate that the higher poly(ADP-ribosyl)ation capacity of long-lived species might more efficiently help to slow down the accumulation of unrepaired DNA damage and of genetic alterations, as compared with short-lived species.

Similar articles

See all similar articles

Cited by 4 articles

References

    1. J Biol Chem. 1981 Apr 25;256(8):3667-70 - PubMed
    1. Mutat Res. 1985 Jan-Feb;142(1-2):69-73 - PubMed
    1. Biochemistry. 1984 Jul 31;23(16):3771-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 May;77(5):2777-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11759-63 - PubMed

Publication types

Substances

LinkOut - more resources

Feedback