Predominant V-region gene configurations in the human antibody response to Haemophilus influenzae capsule polysaccharide

Scand J Immunol. 1995 Apr;41(4):324-30. doi: 10.1111/j.1365-3083.1995.tb03574.x.

Abstract

The antibody response to Haemophilus influenzae type b polysaccharide (Hib PS) is known to be encoded by a few V-region genes. We have obtained four human monoclonal Hib PS antibodies from four healthy adult subjects immunized with diphtheria toxin-conjugated Hib PS vaccine. The VH gene segments that encode for these antibodies belong to the VH3 gene family, of which two are related to the V3-23 gene and two to the VH3b subfamily. Both hybridomas that express a V3-23-related gene use short D-segments (3 bp), the JH6 gene segment and a V kappa gene derived from the A2 germline gene. The two hybridomas that express VH3b genes use D-segments of conventional length (24-33 bp), the JH4 gene segment and a non-A2 V kappa gene. Comparison of our sequences with those reported by others suggests that the above patterns of V-region gene segment association exemplify two V-region gene configurations that are predominant in the Hib PS antibody response. The first configuration is reminiscent of antibodies produced by B-1 B cells while the second is more characteristic of antibodies produced by conventional B cells. The possibility that these two configurations, in fact, represent the products of two different B cell lineages remains to be elucidated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Viral / genetics*
  • Antibodies, Viral / immunology
  • B-Lymphocytes / immunology
  • Base Sequence
  • Diphtheria Toxoid / immunology*
  • Genes, Immunoglobulin / genetics*
  • Genes, Immunoglobulin / immunology
  • Haemophilus Vaccines / immunology*
  • Humans
  • Hybridomas / immunology
  • Immunoglobulin Variable Region / genetics*
  • Mice
  • Molecular Sequence Data
  • Vaccines, Conjugate / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Diphtheria Toxoid
  • Haemophilus Vaccines
  • Haemophilus influenzae type b-polysaccharide vaccine-diphtheria toxoid conjugate
  • Immunoglobulin Variable Region
  • Vaccines, Conjugate