Helicobacter pylori causes hyperproliferation of the gastric epithelium: pre- and post-eradication indices of proliferating cell nuclear antigen

Am J Gastroenterol. 1993 Nov;88(11):1870-5.


Objective: To assess the effect of Helicobacter pylori (HP) eradication on the proliferation of the gastric epithelium by the expression of the proliferating cell nuclear antigen (PCNA).

Methods: Alcohol-fixed gastric biopsies taken before and after treatment for HP were immunostained with the PC-10 anti-PCNA monoclonal antibody and the labeling index was determined with an image analysis system.

Results: The mean PCNA-labeling index (LI) of 16 patients who remained HP positive did not change significantly (18.95 +/- 1.71 on first visit vs. 17.96 +/- 1.91 on second visit, mean +/- SEM). The mean PCNA LI of 31 patients who cleared HP was reduced significantly (19.95 +/- 1.77 on first visit vs. 14.13 +/- 1.29 on second visit, p < 0.001). Patients who were positive for HP at both first and second visit showed a significantly higher PCNA LI than normal control biopsies (13.05 +/- 1.70) (p < 0.05).

Conclusions: These results indicate that the gastric mucosa infected with HP is in a state of hyperproliferation. Patients who cleared HP showed a significant histopathological improvement, reflected in the reduction in number of polymorphonuclear and mononuclear cell infiltrates, and a reduction in the amount of superficial mucosa damage. Prolonged hyperproliferation of the gastric epithelium exerted by HP infection could be a major factor for human gastric carcinogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoantigens / analysis*
  • Biopsy
  • Cell Division / physiology
  • Drug Therapy, Combination
  • Female
  • Gastric Mucosa / microbiology
  • Gastric Mucosa / pathology*
  • Helicobacter Infections / drug therapy*
  • Helicobacter Infections / pathology
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Male
  • Middle Aged
  • Nuclear Proteins / analysis*
  • Proliferating Cell Nuclear Antigen
  • Risk Factors
  • Stomach Neoplasms / epidemiology


  • Autoantigens
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen