Establishment of islet-specific T-cell lines and clones from islet isografts placed in spontaneously diabetic NOD mice

J Autoimmun. 1993 Oct;6(5):517-27. doi: 10.1006/jaut.1993.1043.

Abstract

One impediment to detailed characterization of islet-specific T cells from the NOD mouse model of diabetes is the difficulty encountered in isolation of such cells. This report describes a method that allows routine isolation of relatively large numbers of T cells highly enriched for reactivity toward islet antigens. The method involves renal subcapsular transplantation of spontaneously diabetic NOD mice with NOD islets. These grafts are rapidly destroyed in a tissue-specific manner and this destruction is accompanied by lymphocytic infiltration. Here we demonstrate that the islet graft infiltrates are an excellent source of islet-specific T cells and that islet-specific T-cell lines and clones can be established from these cells. Islet-specific T-cell clones isolated from a T-cell line established from the islet graft infiltrates were capable of adoptive transfer of diabetes to NOD/LtSz-scid recipients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / physiology
  • Cell Line
  • Clone Cells
  • Diabetes Mellitus, Type 1 / immunology*
  • Islets of Langerhans / immunology*
  • Islets of Langerhans Transplantation / immunology*
  • Mice
  • Mice, Inbred NOD
  • T-Lymphocytes / physiology*