Characterization of chromosome 9 deletions in transitional cell carcinoma by microsatellite assay

Hum Mol Genet. 1993 Sep;2(9):1407-11. doi: 10.1093/hmg/2.9.1407.

Abstract

A panel of 18 superficial or invasive transitional cell carcinomas (TCCs) was analyzed for chromosome 9 deletions by performing a high-density loss of heterozygosity (LOH) analysis. Twenty-five microsatellite loci were assayed by the polymerase chain reaction (PCR) and 7 restriction fragment length polymorphism (RFLP) loci were analyzed by Southern blotting. Concordant results were obtained with these methods, including direct comparisons at 2 loci. Chromosome 9 LOH was observed in 13 (72%) of 18 informative cases, including 10 superficial lesions. In contrast, LOH on chromosomes 10, 15, 20 and 21 was < or = 8%. Evidence for missing copies of chromosome 9 was observed in 7 of 13 LOH cases. Comparison of cases with subchromosomal LOH implicated the region between the D9S55 locus on 9p12 and the argininosuccinate synthetase (ASS) locus on 9q34.1 as the location of a tumor suppressor gene relevant to TCC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Argininosuccinate Synthase / genetics
  • Base Sequence
  • Carcinoma, Transitional Cell / genetics*
  • Chromosome Deletion*
  • Chromosomes, Human, Pair 9*
  • DNA Primers / genetics
  • DNA, Neoplasm / genetics
  • DNA, Satellite / genetics
  • Genetic Markers
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Urinary Bladder Neoplasms / genetics*

Substances

  • DNA Primers
  • DNA, Neoplasm
  • DNA, Satellite
  • Genetic Markers
  • Argininosuccinate Synthase